Cell Death Techniques, Taschenbuch von Johnstone, Ricky W. (EDT); Silke, John (E...

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Book Title
Cell Death Techniques
ISBN
9781621820055
Kategorie

Über dieses Produkt

Product Identifiers

Publisher
Cold Spring Harbor Laboratory Press
ISBN-10
162182005X
ISBN-13
9781621820055
eBay Product ID (ePID)
17038799119

Product Key Features

Number of Pages
514 Pages
Publication Name
Cell Death Techniques: a Laboratory Manual
Language
English
Subject
Life Sciences / Cell Biology, Life Sciences / Biochemistry, Life Sciences / Human Anatomy & Physiology
Publication Year
2015
Type
Textbook
Author
John Silke
Subject Area
Science
Format
Trade Paperback

Dimensions

Item Height
1.1 in
Item Weight
0.1 Oz
Item Length
10.7 in
Item Width
8.5 in

Additional Product Features

Intended Audience
Scholarly & Professional
LCCN
2015-020016
Illustrated
Yes
Edition Description
Lab Manual
Synopsis
Cell death plays a critical role in development, normal physiology and many diseases, including cancer. Research in this area is rapidly advancing and involves a variety of specific techniques. This manual provides a step-by-step guide to these techniques, along with background information informing researchers when they should be used., Regulated cell death, which is involved in nearly every aspect of animal development and physiology, can be challenging to study in the laboratory. There are multiple types of cell death, several interconnected signaling pathways can be activated, and the postmortem consequences may vary. Therefore, a combination of specialized techniques is often required to characterize and confirm cell death in the system of interest. This laboratory manual provides a comprehensive suite of step-by-step protocols for inducing, detecting, visualizing, characterizing, and quantifying cell death in a variety of systems. The contributors describe strategies for identifying and evaluating the biochemical and morphological changes that occur during apoptosis, autophagy, necrosis, and other forms of cell death, as well as for pinpointing the genes and proteins that may be involved. The protocols employ flow cytometry, imaging and microscopy, RNAi, western blotting, enzymatic activity measurements, and other tools to assess phenomena such as DNA fragmentation, lysosomal membrane permeabilization, autophagic flux, apoptosome or ripoptosome formation, caspase activation, and cytochrome c release. Techniques are described for both in vitro systems and in vivo models, including mice, C. elegans, and Drosophila. The authors also provide guidance on interpreting and presenting the results of cell death experiments, as well as advice on complementary procedures that may be required to confirm the results of a given experiment. This indispensable laboratory resource will enable all cell and developmental biologists, from the graduate level upward, to confidently carry out and comprehend a wide array of cell viability assays., Regulated cell death, which is involved in nearly every aspect of animal development and physiology, can be challenging to study in the laboratory. There are multiple types of cell death, several interconnected signaling pathways can be activated, and the postmortem consequences may vary. Therefore, a combination of specialized techniques is often required to characterize and confirm cell death in the system of interest. This laboratory manual provides a comprehensive suite of step-by-step protocols for inducing, detecting, visualizing, characterizing, and quantifying cell death in a variety of systems. The contributors describe strategies for identifying and evaluating the biochemical and morphological changes that occur during apoptosis, autophagy, necrosis, and other forms of cell death, as well as for pinpointing the genes and proteins that may be involved. The protocols employ flow cytometry, imaging and microscopy, RNAi, western blotting, enzymatic activity measurements, and other tools to assess phenomena such as DNA fragmentation, lysosomal membrane permeabilization, autophagic flux, apoptosome or ripoptosome formation, caspase activation, and cytochrome c release. Techniques are described for both in vitro systems and in vivo models, including mice, C. elegans , and Drosophila . The authors also provide guidance on interpreting and presenting the results of cell death experiments, as well as advice on complementary procedures that may be required to confirm the results of a given experiment. This indispensable laboratory resource will enable all cell and developmental biologists, from the graduate level upward, to confidently carry out and comprehend a wide array of cell viability assays.
LC Classification Number
QH671.C429 2015

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